Updated on November 21, 2021
A General Introduction Of Paraffin Embedded Tissue Blocks
Paraffin-embedded tissue block usually refers to as FFPE is tissue samples that have been used in many therapeutic applications and research over the past decades. This is a way to store and develop biospecimens for utilization in research, testing, diagnosis, and remedial advancement.
To preserve the tissue sample, it is first fixed with formalin or formaldehyde. This ensures that vital structures and proteins remain the same as when the tissue is still in the host. It is then embedded in a paraffin wax block to facilitate sectioning and mounting onto microscopic slides for examination. To know more about paraffin-embedded tissue visit https://www.geneticistinc.com/blog/paraffin-embedded-tissue-what-is-it for further research in the medical field.
Image Source: Google
Firstly, the tissues are obtained from fresh and unhealthy hosts. Oncologists often relate the primary tumor to samples received from distant metastatic sites in cancer biospecimens. The tissue measured only a few centimeters, depending on the source and tissue. The biospecimen is then immersed in 10% neutral buffered formin for 18-24 hours to harden it.
The tissue is then dried and then cleared with increasing concentrations of ethanol. It is then embedded in immunohistochemistry-grade paraffin, which is used specifically for embedding formalin-fixed tissues. The time it takes to fix tissues is crucial. It can make them unusable for molecular biological studies. It is important to allow for sufficient time for fixation to preserve the tissue. To preserve quality, samples must be handled with care once they have been obtained.
The following are some of the most common therapeutic areas where tissue samples are used: